Impairments of mecA gene detection in bovine Staphylococcus spp.
نویسندگان
چکیده
منابع مشابه
Impairments of mecA gene detection in bovine Staphylococcus spp.
Staphylococcus aureus antimicrobial resistance, especially to beta-lactams, favors treatment failures and its persistence in herd environment. This work aimed to develop a more specific primer for mecA gene detection based on the comparison of the conserved regions from distinct host origins and also investigated the presence of homologue mecA(LGA251) in bovine strains. A total of 43 Staphyloco...
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Background: Among the bacteria that cause food poisoning, Staphylococcus aureus is one of the most common causes of food poisoning worldwide. The aim of this study was to investigate the presence of S. aureus strains in Iranian hamburgers, analysis of their antibiotic resistance pattern, and molecular detection of mecA gene in isolated strains. Methods: A total of 100 Iranian handmade (traditi...
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background staphylococcus aureus is a major pathogen in hospital setting and in the community and causes a wide range of diseases. mrsa infection has recently become a serious problem in anti-microbial chemotherapy. the aim of the study was to detect and analyze the antibiotic diversity and isolation of methicillin resistance gene (meca) of s. aureus isolated from tehran hospitals as a rapid an...
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Methicillin-resistant Staphylococcus aureus has emerged as a serious threat to public health, causing both hospital and community-associated infections. The gold standard for MRSA detection is the amplification of the mecA gene that codes for the production of the altered penicillin-binding protein (PBP2a) responsible for classical methicillin resistance. This work determined the nature of meth...
متن کاملDevelopment of enzyme-labeled oligonucleotide probe for detection of mecA gene in methicillin-resistant Staphylococcus aureus.
A DNA hybridization method with an enzyme-labeled oligonucleotide probe (mecA-ELONP) was developed to detect the methicillin-resistant gene (mecA) in methicillin-resistant Staphylococcus aureus. For rapid identification, bacterial colonies were transferred from agar plates directly onto nylon membranes. Lysis of cells, denaturation of DNA, and hybridization were performed on the membranes. Thes...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2014
ISSN: 1517-8382
DOI: 10.1590/s1517-83822014000300041